What is the purpose of adding the Polyethylene glycol (PEG) to the mixture of cells?

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What do you think would be the action of PEG at the molecular level to accomplish this desired outcome?

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This is an interesting question and one which was eagerly researched in the 1980s by numerous groups. At the time, several mechanisms were suggested for how PEG accomplished cell fusion.  These included

(1) increasing surface tension,
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PEG reduces the zeta potential. Reducing the zeta potential allows the cells to come closer together increasing the chance of their membranes interacting.
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There aren't any perfect teaching strategies as much as there aren't any bad strategies.

Using different methods depending on the topic learned, the class ability to learn each topic, the time allocated, the goals expected, etc. is best for the teacher as much as for the students. Some topics need to be memorized (rote learning) others need to be analyzed and/or experienced (student-centered approach).

In addition, some students learn better by seeing images (visual learner), others learn better by listening (auditory learners) and some need hands-on experience (Kinesthetic learners). Using different methods helps all learners types to learn and understand effectively.
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